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2022-11-20

DEG principle

首先标准化这个数据:计算每一列的size factors,然后normalized;估计基因的离散度,并缩小这些估计值以生成更准确的离散度估计,从而对reads count进行建模;拟合负二项分布的模型,并使用Wald检验或似然比检验进行假设检验。

标准化标准:http://www.360doc.com/content/18/1007/19/51784026_792756336.shtml

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